Objective. Development of themicrobiological diagnosis of urinary tract infections (UTIs), collection and analysis of data on the spectrum of urine microflora and monitoring of its antibiotic sensivity in 2012—2020.

Material and methods. The material for the study was the urine of patients with UTI, pure cultures of gram-negative and gram-positive bacteria isolated by the bacteriological method, the results of testing the antibiotic sensitivity of cultures by agar method diffusion.

Results. 9-year monitoring of the species composition of bacteria associated with UTI has established a high frequency of isolation of E. coli, K. pneumoniae, P. aeroginosa, A. baumanii, Enterococcus spp., Streptococcus spp., Staphylococcus spp., Citrobacter spp., Proteus spp. In the period from 2012 to 2020 the amount of information entered into the computer database about the studied strains of different types of bacteria increased from 2 to 6 times. Monitoring of antibiotic resistance of bacteria for this period showed that the used antimicrobial drugs (cephalosporins of the 3rd generation, carbapenems) resistance of the studied bacteria is high and is characterized by slow changes in dynamics (increase/decrease) in relation to a number of antibiotics or remains relatively stable. The strains of E. coli are characterized by the least resistance, and Klebsiella, Pseudomonas and Acinetobacter are the most resistant. Staphylococci are characterized by a high percentage of methicillin-resistant strains.

Conclusion. Gram-negative (E. coli, Klebsiella, Pseudomonas, Acinetobacter) and gram-positive bacteria (Enterococci, Streptococci, Staphylococci) are detected in the urine of patients with UTI with a high frequency. UTI pathogens are characterized by a high level of the 3rd generation cephalosporin, carbapenems and other antibiotics resistance. Long-term monitoring of bacterial antibiotic resistance indicates about gradual reduction ratio of resistant strains and makes it possible to increase the effectiveness of laboratory diagnostics of uroinfections, to assess the level of resistance of pathogens and the adequacy of the applied antibacterial therapy.