Objective. Multilocus sequence-typing and phylogenetic analysis of 60 meningococci isolated in Belarus between 2011—2018.
Materials and methods. House-keeping genes sequencing was made after Sanger on Applied Biosystems 3500
(52 / 60) and using NGS method on Illumina Miseq (8 / 60).
Results. 60 Belarusian meningococci isolated from patients and carriers for 8 years were divided into 42 sequence-types, 31 of them registered only in the country. ST-11 and ST-9300 (10% each) were the dominant sequence types. In addition, sequence-types circulating in neighboring countries (Russia and Poland) as well as in the European Union members (United Kingdom, Czech Republic, Norway, the Netherlands) circulated in our country. 54.7% of meningococci did not form CC while the remaining ones distributed as follows: CC-103 (20%), CC-11 (10%), CC-41/44 (8.3%), CC-1,
CC-18, CC-32, CC-92 (3.3% each), CC-1136, CC-213, CC-35, CC-364 (1.7% each). MenA meningococci were associated with CC-1, MenB — with CC-103 and CC-32, MenC — with CC-41/44, MenW — with CC-11, MenY — with CC-92.
Conclusion. Belarusian meningococcal population is diverse and includes both its own sequence types (66.7%) and those circulating in other countries (33.3%).Ключевые слова:
Автор(ы): H. N. Kharkhal, L. P. Titov