Objective. To develop a method of cultivation of Toxoplasma gondii in vitro in a laboratory.

Materials and methods. The method of cultivation was developed using the primary cell culture derived from the spleen of mongrel mice and the Toxoplasma gondii invasive culture. A suspension of living cells of the mice spleen was prepared followed by incubating the suspension of cells of the primary culture of the spleen and Toxoplasma gondii invasive culture. A monolayer of cells of the primary culture of the spleen containing Toxoplasma gondii tachysoites was obtained. After the monolayer of spleen cells incubation with Toxoplasma, it was separated, washed, and the number of the Toxoplasma gondii tachyzoites was counted. The Toxoplasma gondii culture in vitro was used for biological and medical experiments aimed at studying the molecular genetic aspects in the modeling of acute, chronic, and congenital toxoplasmosis, developing therapy regimens taking into account nanotechnologies.

Results. In the result of cultivating the Toxoplasma gondii invasive culture using the primary cell culture obtained from the spleen of mongrel mice it is possible to obtain the parasitic culture desired amount in the laboratory for studies of different directions.

Conclusion. Appliance of the method cultivation of the Toxoplasma gondii invasive culture cultivation in vitro in the laboratory will allow constant access to the parasitic material for its experimental use.