THE REPARATIVE EFFECT OF MESENCHYMAL STEM CELLS DURING MODULATION OF CANNABINOID CB2-RECEPTORS IN A MODEL OF PERIPHERAL NEUROPATHY

Objective. To study morphological changes in the distal segment of the sciatic nerve upon modulation of cannabinoid CB2 receptors of mesenchymal stem cells in peripheral neuropathy model.

Materials and methods. Peripheral neuropathy was modeled by excision of a section of the sciatic nerve in Wistar rats. On the 7th day of the experiment, mesenchymal stem cells derived from the rat’s adipose tissue were injected into the site of nerve injury at a dose of 1106 cells/kg with no additional impacts, with preliminary incubation with the CB2 receptor agonist AM1241 or the selective antagonist AM630. On the 21st and 90th days of the experiment, a histological assessment of the distal segment of the sciatic nerve was performed.

Results. It was found that activation of CB2-receptors by the selective agonist AM1241 in the soft tissues of the area of sciatic nerve injury, as well as on mesenchymal stem cell’s membranes, was accompanied by a decrease in the number of damaged nerve fibers and proliferation of Schwann cells in the distal segment of sciatic nerve by the 90th day of the experiment. Inhibition of CB2-receptors by a selective AM630 antagonist was characterized by suppression of the reparative effect of mesenchymal stem cells.

Conclusion. The data obtained indicate the role of cannabinoid CB2 receptors in the implementation of the regenerative effects of mesenchymal stem cells.

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Автор(ы): A.-M. V. Yerofeyeva, S. N. Rjabceva, A. Y. Molchanova