Objective. Determine the genotypes/subgenotypes/subtypes of hepatitis B, C and HIV viruses detected in healthcare workers.

Materials and methods. Serum/plasma, enzyme-linked immunosorbent assay (ELISA), polymerase chain reaction (PCR), reverse transcription polymerase chain reaction (RT-PCR), sequencing, phylogenetic analysis.

Results. Of the 108 sequenced HBV DNA samples 93 (86.1%) belonged to the D genotype of the virus and 15 (13.9%) belonged to A. The performed phylogenetic analysis made it possible to establish that out of 108 sequenced samples in 50.9% (n=55) the subgenotype D2 was detected, in 18.5% (n=20) — D3, in 14.8% (n=16) — D1, in 13.9% (n=15) — A2 and in 1.8% (n=2) cases the D4 subgenotype of the virus was detected. All samples of subgenotype A2 belonged to adw2 HBV serotype, 14 out of 15 sequenced samples of HBV D1 subgenotype belonged to serotype ayw2, and one (JM_1079), obtained from the Mogilev region from a nurse in the treatment room, belonged to ayw4, out of 53 sequenced samples of HBV D2 51 subgenotype belonged to serotype ayw3, one sample was assigned to ayw4 (JM_1079_Mg) and to ayw2 (JM_839_MnObl), out of 20 samples of HBV D3 subgenotype two samples (JM_863_MnObl and JM_19_BrObl) were assigned to ayw1 HBV serotype and onе (JM_869_VitObl) — to ayw4; all the others viruses were assigned to ayw2 HBV serotype.

Of the 43 sequenced HCV samples 35 (81.4%) samples belonged to subgenotype 1b, 6 (14.0%) — to 3a and 2 (4.6%) — to 2a.

Conclusion. Based on the comparative data performed by the method of phylogenetic analysis, it can be concluded that among medical workers virus variants that are not related to each other are mainly circulating. At the same time genetically related isolates of the virus are identified, indicating the presence of a common source of infection, which requires an epidemiological investigation in each case in order to improve the epidemiological surveillance system and to reduce the occurrence of new infections.